Frozen sections amyloid (Amyloid) Thioflavin T (thioflavine T) fluorescent staining kit
Product Manual
The main purpose
YIJI Frozen Section Amyloid (AMYLOID) Thioflavin T fluorescent staining reagent is an authoritative and classic method for analyzing the phenomenon of apple green fluorescence in frozen tissue sections by using Thioflavin T staining technology combined with amyloid protein. Technical method. The technology has been carefully developed and successfully tested. It is mainly suitable for the detection of amyloid in frozen tissue sections. It is widely used in the study of the pathophysiology of amyloid-like diseases. The product is strictly sterile, ready to use, simple in operation, stable in performance and clear in fluorescence.
technical background
Amyloid (AMYLOID) is a homogenous, eosinophilic, insoluble fibrous protein aggregate with a common β-sheet structure and deposited in cells. outer. Protein misfolding, and abnormal self-aggregation will lead to amyloidosis, cytotoxic fiber deposition, tissue damage, resulting in 23 different amyloid syndromes, more than 400 diseases, including Azheim Disease, Parkinson's disease, type 2 diabetes, cataract, prion diseases, etc. Amyloid plaque and neurofibrillary tangles (NFT) are pathological features of amyloid syndrome. Thioflavin T, a benzothiazole derivative, which is formed by methylation of dehydrothiotoluidine with the participation of hydrochloric acid, is the most commonly used amyloid fluorescent probe. , in vivo or in vitro identification display and quantitative analysis of fibrosis kinetics. Thioflavin T is more stable and broad-spectrum than thioflavine S. It is better in vivo and is especially suitable for detection in liquid state. Once bound to amyloid fibers (oligomers), the fluorescence intensity (excitation wavelength 440 nm, emission wavelength 490 nm) is significantly enhanced, showing apple green fluorescence.
product content
YIJI cleaning solution (Reagent A) ml
YIJI Fixative (Reagent B) ml
YIJI staining solution (Reagent C) ml
YIJI Reinforced Liquid (Reagent D) ML
Product manual 1 copy
storage method
Store YIJI staining solution (Reagent C) in a refrigerator at -20 °C, and store the rest in a 4 °C refrigerator; YIJI staining solution (Reagent C) to avoid light; YIJI fixative (Reagent B) is volatile, pay attention to sealing; Corrosive, pay attention to operational safety; effective guarantee for March
User-supplied
(Confocal) fluorescence microscopy: used for observation and analysis of tissue cells after staining
Experimental procedure
- Take 10 pieces of frozen tissue to be tested
- Carefully add xx μl of YIJI Cleaner ( Reagent A ) on the slice to cover the entire surface of the sample.
- Carefully remove the YIJI cleaning solution on the slice ( Reagent A )
- Carefully add xx l YIJI fixing solution (Reagent B) in the slice, covered the entire surface of the slice sample
- Incubate for 10 minutes in 4 refrigerators
- Carefully remove the slices YIJI fixing solution (Reagent B)
- Carefully add xx μl of YIJI Cleaner ( Reagent A ) on the slice to cover the entire surface of the sample.
- Carefully remove the YIJI cleaning solution on the slice ( Reagent A )
- Carefully add xx l YIJI staining solution (Reagent C), covered the entire surface of the slice sample
- Incubate for 10 minutes at room temperature to avoid light
- Carefully remove YIJI staining solution (Reagent C) on the slice
- Carefully add xx μl of YIJI Cleaner ( Reagent A ) on the slice to cover the entire surface of the sample.
- Incubate for 1 minute at room temperature
- Carefully remove the YIJI cleaning solution on the slice ( Reagent A )
- Carefully add xx microliters of YIJI fortification ( Reagent D ) to cover the entire surface of the sample.
- Incubate for 10 minutes at room temperature
- Carefully remove the YIJI fortification ( Reagent D ) on the slice
- Carefully add xx μl of YIJI Cleaner ( Reagent A ) on the slice to cover the entire surface of the sample.
- Incubate for 3 minutes at room temperature
- Carefully remove the YIJI cleaning solution on the slice ( Reagent A )
- Cover
- Immediately under (confocal) fluorescence microscopy (excitation wavelength 440 nm, emission wavelength 490 nm):
Amyloid: showing apple green fluorescence
Precautions
- This product is 50 operations
- Gloves must be worn during operation
- Reagents are corrosive, pay attention to operational safety
- When the reagent solution is on the surface of the sample, avoid the presence of air bubbles and ensure that the sample surface is covered.
- Immediate observation of tissue microscopy after fluorescence microscopy
- Quercetin, curcumin, resveratrol, etc. interfere with detection
- The company provides a series of amyloid staining reagent products.
Quality Standard
- This product has been certified to be stable.
- This product has been identified as fluorescent
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