Release date: 2018-06-19
On June 18th, the research team of Academician Qiao Jie of Peking University Third Hospital and the research team of Professor Tang Fuyue of Peking University School of Life Sciences collaborated and published humans online in the journal Nature Cell Biology (IF=20.06). New advances in pre-implantation multi-omics research. The title is "Single-cell Multi-omics Sequencing of Human Early Embryos". Using the world's leading single-cell COOL-seq technology, the study first mapped the genome-wide DNA methylation and chromatin status of human preimplantation embryos at the single cell level, further analyzing the complex and coordinated embryonic developmental stages. Epigenetic reprogramming process.
The team of Academician Qiao Jie of Peking University Third Hospital has been working closely with the team of Professor Tang Fuyue of Peking University to develop the mechanism of gene expression and epigenetic regulation during human development, and to map the high precision of human preimplantation embryos. Single-cell transcriptome map (Nature Structural & Molecular Biology, 2013), a systematic study of DNA methylation group reprogramming during human early embryo and primordial germ cell development (Nature, 2014; Cell, 2015) Understanding the molecular mechanisms of human embryonic development and gametogenesis provides an important basis. In January 2018, the cooperative team conducted a more in-depth analysis of the preimplantation embryo development process, revealing the dynamic changes of DNA demethylation and de novo methylation in human early embryos and differential methylation of parental source genomes. Key features (Nature Genetics, 2018).
This study aims to further reveal the dynamic remodeling process of chromatin status during DNA methylation reprogramming, parental-specific chromatin status, and the relationship between multi-omics. By using single-cell COOL-seq, euploid and aneuploid cells were distinguished, and single-cell data of euploid embryos were used to systematically depict human preimplantation embryos. Dynamic changes at multiple levels of the epigenome at multiple critical stages during development deepen understanding of the epigenetic reprogramming process in human embryos.
This study reveals for the first time the asymmetric distribution of DNA methylation and chromatin status in the parental genome during human early embryo development. In addition, the study quantitatively compared the chromatin status of human and mouse embryos and found epigenetic features of species conservation and specificity. This provides a theoretical basis for the future study of the similarities and differences of epigenetics in the early embryos of human and mouse species, and clarifies the advantages and limitations of using mouse as a model organism to study the early development of mammals. This study also provides new ideas and research methods for exploring the occurrence mechanism and diagnosis and treatment strategies of clinical embryo development block, implantation failure, and repeated abortion.
Dr. Li Lin, Ph.D. student of Peking University Third Hospital and Ph.D. student of Peking University Beijing Future Gene Diagnosis Center, Ph.D., Gao Yun, researcher Guo Fan of Sichuan University, the first author of the paper; Peking University Third Hospital Qiao Academician Jie and Professor Tang Fuzhong of Peking University School of Life Sciences are co-authors of the paper. The research was funded by the National Natural Science Foundation of China, the National Major Scientific Research Program, the Beijing Municipal Science and Technology Commission, the Beijing Future Gene Diagnosis Advanced Innovation Center, and the Life Sciences Joint Center (CLS).
Source: Peking University Third Hospital
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